WY14643对小鼠早期胚胎抗氧化损伤的影响

doi:10.11843/j.issn.0366-6964.2014.04.006

Abstract

Abstract:

The objective of this experiment was to study the effect of WY14643 on the development of early embryos and to explore the mechanisms of antioxidative damage capacity in early mouse embryos.The model of oxidative damage was established by H₂O₂ and added WY14643 to the medium of in vitro culture.H₂O₂ level in embryos were measured by DCHFDA .SOD activities and MDA contents were determined by using superoxide dismutase and malondialchehyche assay kit，respectively.The results showed that:(1)after different concentrations of WY14643 processing to 2 h，4-cell rate and blastocyst rate in the group of 0.1 μmol•L^-1 WY14643 treatment was significantly higher than other groups（P<0.05）;(2)after 0.1 μmol•L^-1 WY14643 with H₂O₂ co-culture，the embryo development rate was significantly higher than only H₂O₂ treatment group and control group（P<0.05）.(3)through DCHFDA fluorescence detection，the H₂O₂ level of WY14643 adding group was significantly lower than other groups（P<0.05）.(4)from the SOD activities and MDA contents detection，we found that SOD level of adding WY14643 group was significantly higher than other groups within each period（P<0.05）as opposite to the MDA level were significantly lower than other groups（P<0.05）.Those results indicated that WY14643 could improve the development of embryo by increasing activity of SOD enzymes to reduce lipid damage and then decrease the H₂O₂ level of the embryos in vitro culture.

CLC Number:

S814.8

HU De-bao，ZHANG Ye，ZHANG Ri-xin，ZHANG Bao-xiu，LI Zhong-shu，FANG Nan-zhu. Effects of WY14643 on Antioxidative Damage of Early Mouse Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 547-552.

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Effects of WY14643 on Antioxidative Damage of Early Mouse Embryos

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